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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become noticed in several tumors, which ends up in chromosome instability, and thus may lead to tumorigenesis. This protein was located to affiliate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a crucial role in endorsing cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, have been explained. Two additional splice variants ended up claimed but in-depth nucleotide sequence facts is just not nevertheless offered.
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Blocking peptides are peptides that bind specially to your focus on antibody and block antibody binding. These peptide ordinarily is made up of the epitope identified with the antibody.
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This guideline outlines additional attainable optimization of mobile and immunoassay parameters to guarantee the absolute best outcomes are...
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This information outlines even further possible optimization of cellular and immunoassay parameters to make certain the very best outcomes are...
Antibodies sure to the blocking peptide now not bind on the epitope about the focus on protein. By comparing the staining from your blocked antibody compared to the antibody alone, you can see which staining is unique.
Concentrate on Information and facts Cyclin E belongs towards the hugely conserved cyclin relatives, whose associates are characterized by a remarkable periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation patterns which add to your temporal coordination of every mitotic event. Cyclin E sorts a fancy with and functions for a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates within the G1-S stage boundary and is degraded as cells development by means of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...
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